ywbH

168

holin-like protein

locus: BSU_38320

Molecular weight: 14.12 kDa

pI: 9.59

Protein length: 128 aa

Gene length: 387 bp

function: unknown

product: holin-like protein

essential: no

synonyms: ywbH, ipa-23r, cidA

List of homologs in different organisms, belongs to COG1380 (Galperin et al., 2021)

Gene

Coordinates: 3,933,209  3,933,595

Phenotypes of a mutant

• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' mutant is delayed in pellicle formation [Pubmed|26060272]
• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] [gene|E5E431AC5A7D03EEFF6D694CB25EED1570D739A2|yxaK]-[gene|5A43EDB738641C675D4136269F3638F9B609F238|yxaC] [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' triple mutant is severely delayed in pellicle formation [Pubmed|26060272]
• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' overexpression strain has more robust pellicle formation [Pubmed|26060272]

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The protein

Protein family

• CidA/LrgA family (with [protein|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA], according to UniProt)

Structure

• [AF|P39591]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]

  description: [Pubmed|22383849]

  regulation

  • induced by acetate ([protein|search|YwbI]) [Pubmed|26060272]

  regulatory mechanism

  • [protein|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]: activation, [Pubmed|26060272], in [regulon|protein:CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI regulon]

Biological materials

Mutant

• MGNA-B225 (ywbH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1224 NBRP B. subtilis, Japan]
• BKE38320 ([gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCTCCCTTAT,  downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA
• BKK38320 ([gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCTCCCTTAT,  downstream forward: _UP4_AAGAGAAAGGAGAAAAAGCA

References

• 26060272,15659658,17452642