ywbG

168

holin-like auxilary protein

locus: BSU_38330

Molecular weight: 25.67 kDa

pI: 9.56

Protein length: 225 aa

Gene length: 678 bp

function: unknown

product: holin-like auxilary protein

essential: no

synonyms: ywbG, ipa-22r

List of homologs in different organisms, belongs to COG1346 (Galperin et al., 2021)

Gene

Coordinates: 3,933,577  3,934,254

Phenotypes of a mutant

• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' mutant is delayed in pellicle formation [Pubmed|26060272]
• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] [gene|E5E431AC5A7D03EEFF6D694CB25EED1570D739A2|yxaK]-[gene|5A43EDB738641C675D4136269F3638F9B609F238|yxaC] [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' triple mutant is severely delayed in pellicle formation [Pubmed|26060272]
• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' overexpression strain has more robust pellicle formation [Pubmed|26060272]

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The protein

Protein family

• YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family (with [protein|5A43EDB738641C675D4136269F3638F9B609F238|yxaC], according to UniProt)

Structure

• [AF|P39590]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]

  description: [Pubmed|22383849]

  regulation

  • induced by acetate ([protein|search|YwbI]) [Pubmed|26060272]

  regulatory mechanism

  • [protein|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI]: activation, [Pubmed|26060272], in [regulon|protein:CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|ywbI regulon]

Biological materials

Mutant

• MGNA-B224 (ywbG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1223 NBRP B. subtilis, Japan]
• BKE38330 ([gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTTACAATTCCGATGAACA,  downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG
• BKK38330 ([gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACTTACAATTCCGATGAACA,  downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG

References

• 26060272