yvrB

168

[wiki|ABC transporter] (membrane protein) for cobalamin

locus: BSU_33170

Molecular weight: 37.34 kDa

pI: 11.07

Protein length: 353 aa

Gene length: 1062 bp

function: uptake of cobalamin

product: [wiki|ABC transporter] (membrane domain)

essential: no

synonyms: yvrB

List of homologs in different organisms, belongs to COG0609 (Galperin et al., 2021)

Gene

Coordinates: 3,402,469  3,403,530

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The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|FecCD subfamily] (according to UniProt)

Structure

• [PDB|4R9U], the ''E. coli'' BtuC-BtuD complex, BtuC shares 40% identity, 71% similarity with YvrB, [Pubmed|25402482]
• [AF|O34451]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]-[gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]-[gene|128EBAE9C14C7BB75173DF912853A6FCE86ABCE4|yvrA]-[gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]

  regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) ([wiki|B12 riboswitch]) [Pubmed|14704351]

  regulatory mechanism

  • B12 riboswitch: RNA switch, upon binding of B12 [Pubmed|14704351], in [regulon|other_regulator:B12 riboswitch|B12 riboswitch]

Biological materials

Mutant

• MGNA-B044 (yvrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1043 NBRP B. subtilis, Japan]
• BKE33170 ([gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACCAATGATATATGCGGCAT,  downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT
• BKK33170 ([gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACCAATGATATATGCGGCAT,  downstream forward: _UP4_CATCGCGGGGGGAGAAGCTT

References

• 10092453,25402482,14704351