yvdK

168

maltose phosphorylase

locus: BSU_34570

Molecular weight: 88.09 kDa

pI: 5.15

Protein length: 757 aa

Gene length: 2274 bp

function: utilization of maltose

product: maltose phosphorylase

essential: no

synonyms: yvdK, malK, mdxK

List of homologs in different organisms, belongs to COG1554 (Galperin et al., 2021)

Gene

Coordinates: 3,549,228  3,551,501

Advertisement

The protein

Catalyzed reaction/ biological activity

• D-maltose + phosphate --> β-D-glucose 1-phosphate + D-glucose (according to UniProt)

Protein family

• glycosyl hydrolase 65 family (single member, according to UniProt)

Structure

• [PDB|1H54] (from Lactobacillus brevis, 56% identity) [pubmed|11587643]
• [AF|O06993]

Biological materials

Mutant

• MGNA-B623 (yvdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1622 NBRP B. subtilis, Japan]
• BKE34570 ([gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTGATTTTCCATTCAT,  downstream forward: _UP4_GGACGATGTGAAAGGAGAAC
• BKK34570 ([gene|5F615BB886EC4F7000AAD42258DB8E47616552DE|yvdK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTGATTTTCCATTCAT,  downstream forward: _UP4_GGACGATGTGAAAGGAGAAC

References

• 16707683,11587643