frlN

168

aminosugar [wiki|ABC transporter] (permease)

locus: BSU_32590

Molecular weight: 32.68 kDa

pI: 5.29

Protein length: 292 aa

Gene length: 879 bp

function: uptake of sugar amines

product: aminosugar [wiki|ABC transporter] (permease)

essential: no

synonyms: frlN, yurN

List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,348,825  3,349,703

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The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|MalFG subfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 66-282) (according to UniProt)

Structure

• [PDB|4TQU] (from Sphingomonas sp., 28% identity) [pubmed|26235029]
• [AF|O32155]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Biological materials

Mutant

• MGNA-A560 (yurN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/560 NBRP B. subtilis, Japan]
• BKE32590 ([gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA
• BKK32590 ([gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA

References

• 23175651,21815947,12618455,10092453,21347729,21398478,26235029,36815589