essB

essB
168

membrane pseudokinase-like protein, part of the type VII protein secretion system [protein|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[protein|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[protein|6007664F3D979B4D8D4662C7F6E5CF2F489268E3|yukB]-[protein|0A38B2E900532C9950DE6E629F5EB21D64E18B55|yueB]-[protein|DA52C4CD130F32734E01A52F2F395127D2260F61|yueC]

locus
BSU_31890
Molecular weight
52.00 kDa
pI
4.68
Protein length
Gene length
function
export of [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]
product
membrane pseudokinase-like protein, part of the type VII protein secretion system
essential
no
synonyms
yukC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4499 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,274,462  3,275,817
Phenotypes of a mutant
loss of secretion of [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE] [Pubmed|24798022]
Visit Sartorius.com Visit Sartorius.com
Advertisement
The protein
Catalyzed reaction/ biological activity
export of [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE] [Pubmed|24828531,23861817]
secretion and delivery of [wiki|LXG domain] toxins [pubmed|34280190]
Protein family
EssB family (single member, according to UniProt)
[wiki|Domains]
N-terminal pseudokinase domain (aa 3 - 206) [pubmed|36154281]
transmembrane domain (aa 220 - 243) [pubmed|36154281]
C-terminal extracellular domain (aa 244 - 384) [pubmed|36154281]
Structure
[PDB|6Z0F] [pubmed|36154281]
[PDB|4ANO] (from Geobacillus thermodenitrificans, corresponds to the cytoplasmic part, aa 2 ... 218, 44% identity) [pubmed|23499020]
[PDB|2YNQ] (from Geobacillus thermodenitrificans, corresponds to the extracellular part, aa 243 ... 384, 40% identity) [pubmed|23499020]
[AF|P71070]
[wiki|Localization]
cell membrane (according to UniProt) [Pubmed|23861817]
Expression and Regulation
Operons
genes
[gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]-[gene|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[gene|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[gene|6007664F3D979B4D8D4662C7F6E5CF2F489268E3|yukB]-[gene|0A38B2E900532C9950DE6E629F5EB21D64E18B55|yueB]-[gene|DA52C4CD130F32734E01A52F2F395127D2260F61|yueC]-[gene|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]
description
[Pubmed|22383849]
regulation
expressed in the stationary phase [Pubmed|23861817]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|23861817], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|50D2A03E2D7B5D461540FD157377E0D37F771888|yukE]→[gene|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]

2024-02-05 22:53:55

Jstuelk

85

BB0E1B9684F16E121BAA022EF986B0285FB41FA7

CDC40F8A7006CF56CF9A6155897E8BD27FFEC7AA

Biological materials
Mutant
MGNA-A626 (yukC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/626 NBRP B. subtilis, Japan]
BKE31890 ([gene|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC,  downstream forward: _UP4_TAACGTGAGACATGAAGCGC
BKK31890 ([gene|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACCTGACATTCAACCAGATC,  downstream forward: _UP4_TAACGTGAGACATGAAGCGC
References
Reviews
19155186,11973144
Original Publications
15576783,22383849,23861817,24798022,23499020,36154281

3FE4A91C7D0B43C8704391F2F9493852B3AED9B0

Page visits: 2264

Time of last update: 2024-03-28 09:12:27

Author of last update: Jstuelk