yuiE

yuiE
168

similar to leucyl aminopeptidase

locus
BSU_32050
Molecular weight
53.49 kDa
pI
4.55
Protein length
Gene length
function
unknown
product
unknown
essential
no
synonyms
yuiE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0260 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,296,417 → 3,297,919
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The protein
Catalyzed reaction/ biological activity
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low (according to UniProt)
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids (according to UniProt)
Protein family
peptidase M17 family (single member, according to UniProt)
Structure
[PDB|3H8E] (from Pseudomonas putida 37% identity) [pubmed|20359484]
[AF|O32106]
Expression and Regulation
Operons
genes
[gene|C5A6D0D68DFF0459FBBF146854A9F6098E9A3BE8|yuiE]
description
[Pubmed|22383849]
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[gene|C5A6D0D68DFF0459FBBF146854A9F6098E9A3BE8|yuiE]

2024-02-28 23:06:53

Jstuelk

31

a87584b8609fd0c50a11393d144e609f63ff2d2a

552AEF45F13DB2E28D873E4A165B68D676B2340B

Biological materials
Mutant
MGNA-B569 (yuiE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1568 NBRP B. subtilis, Japan]
BKE32050 (Δ[gene|C5A6D0D68DFF0459FBBF146854A9F6098E9A3BE8|yuiE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGGCTTACACTCCTAA,  downstream forward: _UP4_TAATGAAAAATCCCTGCTGT
BKK32050 (Δ[gene|C5A6D0D68DFF0459FBBF146854A9F6098E9A3BE8|yuiE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGGCTTACACTCCTAA,  downstream forward: _UP4_TAATGAAAAATCCCTGCTGT
References
Research papers
20359484,32949815

C5A6D0D68DFF0459FBBF146854A9F6098E9A3BE8

Page visits: 1192

Time of last update: 2024-03-27 04:00:17

Author of last update: Jstuelk