ytzG

168

similar to 16S pseudouridylate synthase

locus: BSU_30035

Molecular weight: 27.00 kDa

pI: 5.619

Protein length: 239 aa

Gene length: 720 bp

function: 16S rRNA modification

product: putative 16S pseudouridylate synthase

essential: no

ec: 5.4.99.19

synonyms: ytzG, ytzF

List of homologs in different organisms, belongs to COG1187 (Galperin et al., 2021)

Gene

Coordinates: 3,072,743  3,073,462

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The protein

Catalyzed reaction/ biological activity

• uridine⁵¹⁶ in 16S rRNA --> a pseudouridine⁵¹⁶ in 16S rRNA (according to E. coli RsuA)

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RsuA family (with [protein|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB], according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 1-65) (according to UniProt)

Structure

• [PDB|1KSK] (RsuA from E. coli, 40% identical) [pubmed|11953756]
• [AF|O32068]

Expression and Regulation

Operons

• genes: [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]-[gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE30035 ([gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30035 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA,  downstream forward: _UP4_TGACAAAAACCCCGCATACC
• BKK30035 ([gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30035 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA,  downstream forward: _UP4_TGACAAAAACCCCGCATACC

References

Research papers

• 11953756