mnmM

168

SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase

locus: BSU_30490

Molecular weight: 21.14 kDa

pI: 5.56

Protein length: 194 aa

Gene length: 585 bp

function: tRNA modification at U34

product: SAM-dependent class I 5-aminomethyl-2-thiouridine methyltransferase

essential: no

synonyms: ytqB, MnmM

List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)

Gene

Coordinates: 3,120,964 → 3,121,548

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The protein

Catalyzed reaction/ biological activity

• modification of tRNA at U34, conversion of 5-aminomethyl-(2-thio)uridine to 5-methylaminomethyl-(2-thio)uridine [pubmed|36762482]

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)

[wiki|Cofactors]

• SAM [Pubmed|24637210]

Structure

• [PDB|4PON], apoenzyme [Pubmed|24637210]
• [PDB|4POO], complex with SAM [Pubmed|24637210]
• [PDB|8H1B], [protein|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM] from S. aureus complexed with SAM and tRNA anti-codon stem loop
• [AF|O34614]

Expression and Regulation

Operons

• genes: [gene|AB9762F1D388E27039FEFE946D8BE527CEF13F09|mnmL]-[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A165 (ytqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/165 NBRP B. subtilis, Japan]
• BKE30490 (Δ[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA,  downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC
• BKK30490 (Δ[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTAAGGAAGAATTTTCTTCA,  downstream forward: _UP4_GTCGCCATCGAAAAAAAAGC

References

• 24637210,24699744,36762482