ytnP

168

lactonase-homolog protein, inhibits the signalling pathway required for the streptomycin production and development of aerial mycelium in Streptomyces griseus

locus: BSU_29890

Molecular weight: 29.02 kDa

pI: 5.93

Protein length: 256 aa

Gene length: 771 bp

function: defense against competing bacteria

product: lactonase-homolog protein

essential: no

synonyms: ytnP

List of homologs in different organisms, belongs to COG0491 (Galperin et al., 2021)

Gene

Coordinates: 3,058,631  3,059,401

Advertisement

The protein

Protein family

• [wiki|metallo-beta-lactamase superfamily] (according to UniProt)

Structure

• [PDB|3ESH] (from Staphylococcus aureus, 50% identity)
• [AF|O34760]

Modification

• phosphorylation on Ser-36 [Pubmed|17218307]

Expression and Regulation

Operons

• genes: [gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]-[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A511 (ytnP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/511 NBRP B. subtilis, Japan]
• BKE29890 ([gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCGCCTCCGTCCATAT,  downstream forward: _UP4_TAGCATATTTTTATGTTTAC
• BKK29890 ([gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCGCCTCCGTCCATAT,  downstream forward: _UP4_TAGCATATTTTTATGTTTAC

References

• 17218307,22101040,34073161