polX

168

DNA polymerase X, involved in DNA repair, generates adaptive mutations by error-prone processing of A/P sites

Locus: BSU_28590

Molecular weight: 63.95 kDa

Isoelectric point: 5.31

Protein length: 570 aa

Gene length: 1713 bp

Function: DNA repair

Product: DNA polymerase X

Essential: no

E.C.: 2.7.7.7

Synonyms: polX, yshC

Genomic Context

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List of homologs in different organisms, belongs to COG1796 (Galperin et al., 2021)

Gene

Coordinates: 2,923,314 → 2,925,026

The protein

Catalyzed reaction/ biological activity

• template-dependent DNA polymerase, fills single nucleotide gaps PubMed
• has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates PubMed
• has intrinsic apurinic/apyrimidinic (AP) endonuclease activity PubMed
• 2'-deoxyribonucleoside 5'-triphosphate + DNA_(n) --> diphosphate + DNA_(n+1) (according to UniProt)

Protein family

• N-terminal part: DNA polymerase type-X family (single member, according to UniProt)
• C-terminal part: PHP family (single member, according to UniProt)

Domains

• C-terminal PHP domain with an active site formed by nine histidines and aspartates that catalyzes 3'-5' exonuclease, AP-endonuclease, 3'-phosphodiesterase and 3'-phosphatase activities PubMed

Structure

• 3AU2 (PDB) (from Thermus thermophilus, complex with dGTP, 37% identity) PubMed
• P94544 (AlphaFold)

Expression and Regulation

Operons

• Genes: zapA-yshB-polX-rqcU-yshE

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• GP3560 (Δ(polX-rqcU)::kan), available in Jörg Stülke's lab
• MGNA-B003 (yshC::erm), available at the NBRP B. subtilis, Japan
• BKE28590 (ΔpolX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATAACCCCCAGACG,  downstream forward: _UP4_TAAGTAAGGAGGCTCACACA
• BKK28590 (ΔpolX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATAACCCCCAGACG,  downstream forward: _UP4_TAAGTAAGGAGGCTCACACA

Labs working on this gene/protein

• Margarita Salas, Madrid, Spain link

References

Reviews

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Original Publications

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