ysfE

168

unknown

locus: BSU_28700

Molecular weight: 8.69 kDa

pI: 4.32

Protein length: 80 aa

Gene length: 243 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: ysfE

List of homologs in different organisms, belongs to COG2764 (Galperin et al., 2021)

Gene

Coordinates: 2,935,967  2,936,209

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The protein

[wiki|Domains]

• [wiki|VOC domain] (aa 1-78) (according to UniProt)

Structure

• [AF|P94533]

Expression and Regulation

Operons

• genes: [gene|F93E473C06669DE604EC027236C79E5450F53720|cstA]-[gene|5E483479E65CF5E648A88F3B9029CCE555B27898|ysfE]

  description: [Pubmed|22383849]

  regulation

  • repressed by glucose (3.4-fold) ([protein|search|CcpA]) [Pubmed|12850135,22900538]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22900538], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|5E483479E65CF5E648A88F3B9029CCE555B27898|ysfE]

  description: [Pubmed|8969504]

Biological materials

Mutant

• MGNA-B026 (ysfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1025 NBRP B. subtilis, Japan]
• BKE28700 ([gene|5E483479E65CF5E648A88F3B9029CCE555B27898|ysfE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATCGTAGACCCTGTAT,  downstream forward: _UP4_TAACGCACAAAAGCCCAAAA
• BKK28700 ([gene|5E483479E65CF5E648A88F3B9029CCE555B27898|ysfE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATCGTAGACCCTGTAT,  downstream forward: _UP4_TAACGCACAAAAGCCCAAAA

References

• 8969504,22383849,22900538