glcD

168

probably glycolate oxidase subunit, FAD-binding

locus: BSU_28680

Molecular weight: 50.77 kDa

pI: 4.86

Protein length: 470 aa

Gene length: 1413 bp

function: unknown

product: probably glycolate oxidase subunit

essential: no

ec: 1.1.3.15

synonyms: glcD, ysfC, glcD

List of homologs in different organisms, belongs to COG0277 (Galperin et al., 2021)

Gene

Coordinates: 2,933,185  2,934,597

The protein

Protein family

• FAD-binding oxidoreductase/transferase type 4 family (single member, according to UniProt)

[wiki|Domains]

• [wiki|FAD-binding PCMH-type domain] (aa 37-216) (according to UniProt)

[wiki|Cofactors]

• FAD (acording to UniProt)

Structure

• [PDB|3PM9] (dehydrogenase from Rhodopseudomonas palustris, 32% identity)
• [AF|P94535]

Additional information

• The gene is annotated in KEGG as an ortholog of (S)-2-hydroxy-acid oxidase EC 1.1.3.15. No EC annotation is available in Swiss-Prot. In MetaCyc, it is annotated as similar to glycolate oxidase subunit. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

Expression and Regulation

Operons

• genes: [gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]-[gene|DDD01D9C50BC11A50D263946ECA15CB58570ACAA|glcF]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B001 (ysfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1000 NBRP B. subtilis, Japan]
• BKE28680 ([gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCACCCATCCCCCTGT,  downstream forward: _UP4_AGAAAACGTGTGGTGGCGGA
• BKK28680 ([gene|386BB8A42AF9FEB27FA3CABEDA21AA153A544316|glcD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCACCCATCCCCCTGT,  downstream forward: _UP4_AGAAAACGTGTGGTGGCGGA

References

Research papers

• 27264531,8606183