pftA

168

pyruvate transporter

locus: BSU_28910

Molecular weight: 15.57 kDa

pI: 9.82

Protein length: 146 aa

Gene length: 441 bp

function: uptake of pyruvate

product: pyruvate transporter

essential: no

synonyms: pftA, ysbA, lrgA

List of homologs in different organisms, belongs to COG1380 (Galperin et al., 2021)

Gene

Coordinates: 2,955,209  2,955,649

Phenotypes of a mutant

• strongly impaired growth with pyruvate as the single carbon source [Pubmed|28974613,27422364]
• ''[gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' mutant is delayed in pellicle formation [Pubmed|26060272]
• a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] [gene|E5E431AC5A7D03EEFF6D694CB25EED1570D739A2|yxaK]-[gene|5A43EDB738641C675D4136269F3638F9B609F238|yxaC] [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' triple mutant is severely delayed in pellicle formation [Pubmed|26060272]

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The protein

Catalyzed reaction/ biological activity

• uptake of pyruvate [pubmed|28974613]

Protein family

• CidA/LrgA family (with [protein|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH], according to UniProt)

Structure

• [AF|P94515]

[wiki|Localization]

• cell membrane [pubmed|28974613]

Expression and Regulation

Operons

• genes: [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]

  description: [Pubmed|21815947]

  regulation

  • induced by acetate [Pubmed|26060272]
  • induced by pyruvate ([protein|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]) [pubmed|28974613]
  • subject to carbon catabolite repression ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [pubmed|28974613]

  regulatory mechanism

  • [protein|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]: activation, [Pubmed|28974613,27422364], in [regulon|protein:53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, ([Pubmed|28974613,27422364], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]

  additional information

  • the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon is strongly upregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
  • the [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB] operon is strongly upregulated in a ''[wiki|cshA]'' mutant [Pubmed|23175651]

Biological materials

Mutant

• MGNA-B013 (ysbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1012 NBRP B. subtilis, Japan]
• GP1554 (''[gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]''::''spc''), available in [wiki|Jörg Stülke]'s lab
• BKE28910 ([gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTCACCTCTTTCT,  downstream forward: _UP4_TAAGGGAAACAGGAGGTTCT
• BKK28910 ([gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTCACCTCTTTCT,  downstream forward: _UP4_TAAGGGAAACAGGAGGTTCT

References

Reviews

• 27511628,29208748,29354650

Original Publications

• 26060272,23175651,21815947,15659658,17452642,26110430,27422364,28974613