ragB

168

[protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] glycosyltransferase booster, likely involved in sidewall synthesis during cell growth

locus: BSU_27300

Molecular weight: 25.18 kDa

pI: 4.73

Protein length: 233 aa

Gene length: 702 bp

function: control of [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] activity

product: [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] glycosyltransferase booster

essential: no

synonyms: yrrS

List of homologs in different organisms

Gene

Coordinates: 2,788,920  2,789,621

Phenotypes of a mutant

• an [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] [gene|9892E5BFE90EC934A4047795BC4B328656128C09|ragB] double mutant has a severe growth defect [pubmed|39185233]
• a Δ[gene|9892E5BFE90EC934A4047795BC4B328656128C09|ragB] Δ[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] double mutant has a severe growth defect, this can be suppressed by over expression of [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA] [pubmed|40817173]

The protein

Catalyzed reaction/ biological activity

• may play a role in cell division based on strong correlations to the genetic interaction profile of known cell division genes such as [gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF], [gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL], and [gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC] [pubmed|39185233]

Structure

• [AF|O32031]

[wiki|Localization]

• forms foci in the membrane [pubmed|40817173]

Expression and Regulation

Operons

• genes: [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]-[gene|9892E5BFE90EC934A4047795BC4B328656128C09|ragB]

  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in both cell compartments ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE] [Pubmed|14679248,15758244], [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] [Pubmed|15699190,16497325])

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|14679248,15758244], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|15699190,16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• MGNA-A852 (yrrS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/852 NBRP B. subtilis, Japan]
• BKE27300 ([gene|9892E5BFE90EC934A4047795BC4B328656128C09|ragB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATAATTCTGCACTCCTTT,  downstream forward: _UP4_TAAACAAAAGCAGCCGGGTT
• BKK27300 ([gene|9892E5BFE90EC934A4047795BC4B328656128C09|ragB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATAATTCTGCACTCCTTT,  downstream forward: _UP4_TAAACAAAAGCAGCCGGGTT

References

• 14679248,15699190,16497325,30651563,39185233,40817173