yrkA

168

similar to magnesium exporter

locus: BSU_26610

Molecular weight: 48.82 kDa

pI: 4.92

Protein length: 434 aa

Gene length: 1305 bp

function: unknown

product: unknown

essential: no

synonyms: yrkA

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

Gene

Coordinates: 2,718,959  2,720,263

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The protein

Protein family

• [wiki|UPF0053 family](according to UniProt)

[wiki|Domains]

• [wiki|CNNM transmembrane domain] (aa 1-201) (according to UniProt)
• [wiki|DUF21 domain] (5-201)
• 2 [wiki|CBS domain]s (aa 220-282, aa 289-346) (according to UniProt)

Structure

• [PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
• [AF|P54428]

Paralogous protein(s)

• [protein|A459410312A926365B45CEB4694DE399B38820A2|yugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG,  downstream forward: _UP4_TAACATCTGGATAAAACACA
• BKK26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG,  downstream forward: _UP4_TAACATCTGGATAAAACACA

Expression vectors

• pGP2932 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP3465 (214-350aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP3470 (214-350aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab

References

• 27933050,31415562