forE2

168

essential subunit of [metabolite|formate]:[metabolite|menadione] oxidoreductase [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2], links [metabolite|formate] oxidation to the aerobic respiratory chain

locus: BSU_27230

Molecular weight: 17.37 kDa

pI: 4.76

Protein length: 160 aa

Gene length: 483 bp

function: links [metabolite|formate] oxidation to the aerobic respiratory chain

product: essential subunit of formate:menadione oxidoreductase [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

essential: no

ec: null

synonyms: yrhD

List of homologs in different organisms, belongs to COG2427 (Galperin et al., 2021)

Gene

Coordinates: 2,784,170  2,784,652

The protein

Catalyzed reaction/ biological activity

• acts as a membrane-associate scaffold for [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2], links [metabolite|formate] oxidation to the aerobic respiratory chain [pubmed|40855134]

Protein family

• DUF1641 family (with [protein|8279630BE1D1D214162BA8E06FC05146F9ED517D|yjlC] and [protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1])

Structure

• [PDB|8RQZ] (crystal structure of [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]₄-[protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1]4) [pubmed|40855134]
• [PDB|8RR0] (cryo-EM structure of [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]₄-[protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1]4) [pubmed|40855134]
• [AF|O05396]

[wiki|Localization]

• associated to the membrane [pubmed|40855134]

Expression and Regulation

Operons

• genes: [gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

  description: [Pubmed|21815947]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|search|yrhE] [Pubmed|27120414]

Biological materials

Mutant

• MGNA-A150 (yrhD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/150 NBRP B. subtilis, Japan]
• BKE27230 ([gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGCCATTCGTTTTCCTCCTA,  downstream forward: _UP4_TAACAGAAAAACAGCCATCT
• BKK27230 ([gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGCCATTCGTTTTCCTCCTA,  downstream forward: _UP4_TAACAGAAAAACAGCCATCT

References

• 21815947,34748728,40855134