yrbF

168

part of the Sec protein translocation machinery

locus: BSU_27700

Molecular weight: 9.77 kDa

pI: 9.67

Protein length: 89 aa

Gene length: 267 bp

function: protein secretion

product: Sec translocon accessory complex subunit

essential: no

synonyms: yrbF

List of homologs in different organisms, belongs to COG1862 (Galperin et al., 2021)

Gene

Coordinates: 2,832,424 → 2,832,690

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The protein

Protein family

• YajC family (single member, according to UniProt)

Structure

• [AF|O32052]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]

  description: [pubmed|22383849]

  regulation

  • the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

• genes: [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE27700 (Δ[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTTTCACCCCTTTCT,  downstream forward: _UP4_TAACAGCAGGAGCTATACAT
• BKK27700 (Δ[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTTTCACCCCTTTCT,  downstream forward: _UP4_TAACAGCAGGAGCTATACAT

References

• 36815589,18763711,29794222,16997527,26112883