tlyA

168

2′-O-methylcytidine methyltransferase

locus: BSU_24260

Molecular weight: 29.50 kDa

pI: 10.11

Protein length: 281 aa

Gene length: 846 bp

function: methylation of C1417 in 16S rRNA and C1949 in 23S rRNA

product: rRNA methyltransferase

essential: no

synonyms: yqxC, yqiF

List of homologs in different organisms, belongs to COG1189 (Galperin et al., 2021)

Gene

Coordinates: 2,522,871  2,523,716

The protein

Catalyzed reaction/ biological activity

• methylation of C1417 in 16S rRNA and C1949 in 23S rRNA [pubmed|39036956]

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• TlyA family (single member, according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 6-67) (according to UniProt)

[wiki|Cofactors]

• SAM [pubmed|28031456]

Structure

• [PDB|3HP7] (from Streptococcus thermophilus, 60% identity)
• [AF|P19672]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]

  description: [Pubmed|2507400]

  additional information

  • the terminator is [protein|search|NusA]-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

  description: [pubmed|22383849]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• MGNA-C369 (yqxC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2367 NBRP B. subtilis, Japan]
• BKE24260 ([gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCTAATCGTTCTTTCTTTG,  downstream forward: _UP4_TAATAGGGCGCTATTATTCC
• BKK24260 ([gene|923C4177500E9678A0B4855F8F481D4A36ECD863|tlyA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCTAATCGTTCTTTCTTTG,  downstream forward: _UP4_TAATAGGGCGCTATTATTCC

References

• 2507400,28031456,20854656,21443791,24809820,39036956