tlyA

168

rRNA methyltransferase

Locus: BSU_24260

Molecular weight: 29.50 kDa

Isoelectric point: 10.11

Protein length: 281 aa

Gene length: 846 bp

Function: methylation of C1417 in 16S rRNA and C1949 in 23S rRNA

Product: rRNA methyltransferase

Essential: no

Synonyms: yqxC, yqiF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1189 (Galperin et al., 2021)

Gene

Coordinates: 2,522,871  2,523,716

The protein

Catalyzed reaction/ biological activity

• methylation of C1417 in 16S rRNA and C1949 in 23S rRNA PubMed

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• TlyA family (single member, according to UniProt)

Domains

• S4 RNA-binding domain (aa 6-67) (according to UniProt)

Cofactors

• SAM PubMed

Structure

• 3HP7 (PDB) (from Streptococcus thermophilus, 60% identity)
• P19672 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: tlyA

  Description: PubMed

  Additional information

  • the terminator is NusA-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]

• Genes: dxs-tlyA-ahrC-recN

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

Biological materials

Mutant

• MGNA-C369 (yqxC::erm), available at the NBRP B. subtilis, Japan
• BKE24260 (tlyA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCTAATCGTTCTTTCTTTG,  downstream forward: _UP4_TAATAGGGCGCTATTATTCC
• BKK24260 (tlyA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCTAATCGTTCTTTCTTTG,  downstream forward: _UP4_TAATAGGGCGCTATTATTCC

References

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