yqkF

168

NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

locus: BSU_23620

Molecular weight: 34.56 kDa

pI: 5.13

Protein length: 306 aa

Gene length: 921 bp

function: unknown

product: NADPH-dependent 4-Hydroxy-2,3-trans-nonenal reductase

essential: no

synonyms: yqkF

List of homologs in different organisms, belongs to COG0667 (Galperin et al., 2021)

Gene

Coordinates: 2,459,326 → 2,460,246

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The protein

Protein family

• [wiki|Aldo/keto reductase family] (according to UniProt)
• [wiki|Aldo/keto reductase 2 subfamily] (according to UniProt)

[wiki|Cofactors]

• NADPH [Pubmed|16242712]

Structure

• [PDB|1YNP] (from ''B. halodurans'', 64% identity) [Pubmed|16242712]
• [AF|P54569]

Paralogous protein(s)

• [protein|F1AFFACD36C75702B0BB8F0B4241A9688FB6253C|iolS], (31,4%)

Expression and Regulation

Operons

• genes: [gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]

  description: (according to [http://dbtbs.hgc.jp/COG/prom/yqkF.html DBTBS]) null

• genes: [gene|08EBBA57B807F8C27C37BEB275DF5E206935C698|mciZ]-[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]

  description: [pubmed|22383849]

  regulation

  • expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18284588]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [pubmed|18284588], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE23620 (Δ[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGCACACTCCTTTTTT,  downstream forward: _UP4_TAGCAGAAAAACCAGCACCT
• BKK23620 (Δ[gene|B0897643562CDB25547223CCB9CFD0F9E78710E2|yqkF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGCACACTCCTTTTTT,  downstream forward: _UP4_TAGCAGAAAAACCAGCACCT