brxB

168

bacilliredoxin

locus: BSU_23990

Molecular weight: 16.05 kDa

pI: 4.81

Protein length: 145 aa

Gene length: 438 bp

function: de-bacillithiolation of S-bacillithiolated [protein|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR] and [protein|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|metE]

product: bacilliredoxin

essential: no

synonyms: brxB, yqiW

List of homologs in different organisms

Gene

Coordinates: 2,493,064  2,493,501

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The protein

Catalyzed reaction/ biological activity

• de-bacillithiolation of S-bacillithiolated [protein|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR] and [protein|23F405D5B849E4EDC1D599F86C71DFD2E55C0305|metE] [Pubmed|24313874]

Protein family

• UPF0403 family (with [protein|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA], according to UniProt)

[wiki|Domains]

• contains an CxC redox motif redox active Cys residue [Pubmed|20308541]

Structure

• [PDB|3FHK] ([protein|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA]) [Pubmed|19653655]
• [AF|P54534]

Paralogous protein(s)

• [protein|FBE3C55526FE0CBBDA71AE0B0D7C6AD074E8A63E|brxA]

Expression and Regulation

Operons

• genes: [gene|8B134BDC82C92DB7747A3AF291C96BB0F223D0CF|brxB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C380 (yqiW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2378 NBRP B. subtilis, Japan]
• BKE23990 ([gene|8B134BDC82C92DB7747A3AF291C96BB0F223D0CF|brxB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGAAGACCTCTCCTTTT,  downstream forward: _UP4_TAAGACGAACAACCCGGATG
• BKK23990 ([gene|8B134BDC82C92DB7747A3AF291C96BB0F223D0CF|brxB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGAAGACCTCTCCTTTT,  downstream forward: _UP4_TAAGACGAACAACCCGGATG

References

• 24313874,20308541,19653655