yqhS

168

similar to 3-dehydroquinate dehydratase

locus: BSU_24470

Molecular weight: 16.29 kDa

pI: 5.12

Protein length: 148 aa

Gene length: 447 bp

function: unknown

product: unknown

essential: no

ec: 4.2.1.10

synonyms: yqhS

List of homologs in different organisms, belongs to COG0757 (Galperin et al., 2021)

Gene

Coordinates: 2,539,755 → 2,540,201

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The protein

Catalyzed reaction/ biological activity

• 3-dehydroquinate --> 3-dehydroshikimate + H₂O (according to UniProt)

Protein family

• type-II 3-dehydroquinase family (single member, according to UniProt)

Structure

• [PDB|1GQO]
• [AF|P54517]

Expression and Regulation

Operons

• genes: [gene|449EEBF33F52B461F244331ACD140E68CF64EE7C|figP]-[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

  description: [pubmed|22383849]

• genes: [gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C465 (yqhS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2463 NBRP B. subtilis, Japan]
• CS214 (''[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE24470 (Δ[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACAATCTCTCCTTTTCG,  downstream forward: _UP4_AGCCAGCAAGGGGGAGAATC
• BKK24470 (Δ[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACAATCTCTCCTTTTCG,  downstream forward: _UP4_AGCCAGCAAGGGGGAGAATC

References

• 27197833