yqhB

168

similar to magnesium exporter, general stress protein, required for protection against oxidative stress

locus: BSU_24750

Molecular weight: 49.79 kDa

pI: 5.54

Protein length: 442 aa

Gene length: 1329 bp

function: protection against stress conditions

product: unknown

essential: no

synonyms: yqhB

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

Gene

Coordinates: 2,561,585 → 2,562,913

Advertisement

The protein

Protein family

• [wiki|UPF0053 family](according to UniProt)

[wiki|Domains]

• [wiki|CNNM transmembrane domain] (aa 7-210) (according to UniProt)
• [wiki|DUF21 domain] (14-210)
• 2 [wiki|CBS domain]s (aa 229-290, aa 295-355) (according to UniProt)

Structure

• [PDB|4HG0] (CorC from E. coli, corresponds to the [wiki|CBS domain]s and the C-terminal transporter-associated domain)
• [AF|P54505]

Paralogous protein(s)

• [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|yugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|yhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]

  description: [Pubmed|16267290]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-C474 (yqhB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2472 NBRP B. subtilis, Japan]
• BKE24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA,  downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
• BKK24750 (Δ[gene|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|yqhB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA,  downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC

Expression vectors

• pGP2935 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP3467 (223-359aa) (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP3472 (223-359aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab

References

• 16267290,15805528,22582280,27933050,31415562