trmK

168

tRNA:m1A22 methyl transferase

locus: BSU_25180

Molecular weight: 23.56 kDa

pI: 5.27

Protein length: 216 aa

Gene length: 651 bp

function: tRNA modification

product: tRNA:m1A22 methyl transferase

essential: no

synonyms: trmK, yqfN

List of homologs in different organisms, belongs to COG2384 (Galperin et al., 2021)

Gene

Coordinates: 2,598,616 → 2,599,266

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The protein

Catalyzed reaction/ biological activity

• Catalyzes the S-adenosyl-L-methionine-dependent formation of N₁-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]
• adenosine²² in tRNA + S-adenosyl-L-methionine --> H⁺ + N¹-methyladenosine²² in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]

Protein family

• TrmK family (single member, according to UniProt)

[wiki|Domains]

• N-terminal Class I MTase domain [pubmed|30931478]
• C-terminal coiled-coil domain [pubmed|30931478]

[wiki|Cofactors]

• SAM [pubmed|30931478]

Structure

• [PDB|6Q56] [pubmed|30931478]
• [AF|P54471]

Modification

• phosphorylation on Ser-48 [Pubmed|17218307]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]

  description

  • [pubmed|22383849]

Biological materials

Mutant

• BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
• BKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA

References

• 18420655,17218307,27098549,30931478,38331425