yqeM

168

similar to SAM-dependent methyltransferase

locus: BSU_25610

Molecular weight: 28.18 kDa

pI: 4.26

Protein length: 247 aa

Gene length: 744 bp

function: unknown

product: unknown

essential: no

synonyms: yqeM

List of homologs in different organisms, belongs to COG2226 (Galperin et al., 2021)

Gene

Coordinates: 2,642,101  2,642,844

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The protein

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)

[wiki|Cofactors]

• SAM

Structure

• [PDB|3D2L] (from Exiquobacterium sp., 45% identity)
• [AF|P54458]

Expression and Regulation

Operons

• genes: [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|yqeL]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]

  description: [Pubmed|22383849]

  expression

  • expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

Biological materials

Mutant

• MGNA-C499 (yqeM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2497 NBRP B. subtilis, Japan]
• BKE25610 ([gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCTTGCAAAGCCTTGAT,  downstream forward: _UP4_TAAAACGATGGTTTTTTAAA
• BKK25610 ([gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCTTGCAAAGCCTTGAT,  downstream forward: _UP4_TAAAACGATGGTTTTTTAAA

References

• 22383849