yqcG

168

LXG toxin, eliminates defective cells from developing biofilms, RNase activity

Locus: BSU_25860

Molecular weight: 59.50 kDa

Isoelectric point: 8.81

Protein length: 531 aa

Gene length: 1596 bp

Function: eliminates defective cells from developing biofilms

Product: LXG toxin, RNase

Essential: yes

Synonyms: yqcG

Genomic Context

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List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

Gene

Coordinates: 2,661,102 → 2,662,697

Phenotypes of a mutant

• the mutant is outcompeted by wild type cells in biofilms PubMed

The protein

Catalyzed reaction/ biological activity

• part of a type II toxin/antitoxin system (with YqcF) PubMed
• eliminates defective cells from developing biofilms PubMed
• DNA degradation PubMed

Domains

• LXG domain (aa 1-235) (according to UniProt)

Structure

• P45942 (AlphaFold)

Localization

• secreted
• secretion and delivery requires WXG100 and the T7SS (YukD-EssB-YukB-YueB-YueC-YueD) PubMed

Expression and Regulation

Operons

• Genes: yqcG-yqcF

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon
  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• BKE25860 (ΔyqcG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCATATCCTTTCATT,  downstream forward: _UP4_GGAGATTAAGGAGAGATTGT
• BKK25860 (ΔyqcG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCATATCCTTTCATT,  downstream forward: _UP4_GGAGATTAAGGAGAGATTGT

References

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