ypuI

168

rRNA pseudouridine 2633 synthase

Locus: BSU_23200

Molecular weight: 20.21 kDa

Isoelectric point: 5.49

Protein length: 179 aa

Gene length: 540 bp

Function: translation

Product: rRNA pseudouridine 2633 synthase

Essential: no

E.C.: null

Synonyms: ypuI

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,424,654  2,425,193

The protein

Structure

• P35156 (AlphaFold)

Expression and Regulation

Operons

• Genes: scpA-scpB-ypuI

  Description: PubMed

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

Biological materials

Mutant

• MGNA-A094 (ypuI::erm), available at the NBRP B. subtilis, Japan
• BKE23200 (ypuI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCTTCTTCCTTAT,  downstream forward: _UP4_TAACGTTTACTCTCCCTTTT
• BKK23200 (ypuI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAGCTTCTTCCTTAT,  downstream forward: _UP4_TAACGTTTACTCTCCCTTTT

References

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