mrfA

168

ATP-dependent helicase, repair of mitomycin C-induced DNA lesions, part of a novel nucleotide excision repair pathway

locus: BSU_22220

Molecular weight: 84.40 kDa

pI: 6.16

Protein length: 749 aa

Gene length: 2250 bp

function: repair of mitomycin C-induced DNA lesions

product: 3′-to-5′ DNA helicase

essential: no

synonyms: mrfA, yprA

List of homologs in different organisms, belongs to COG1205 (Galperin et al., 2021)

Gene

Coordinates: 2,334,581  2,336,830

Phenotypes of a mutant

• sensitive to mitomycin C [pubmed|30379365]

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The protein

Catalyzed reaction/ biological activity

• 3′-to-5′ DNA helicase [pubmed|33300032]

Protein family

• [wiki|helicase family] (according to UniProt)

[wiki|Domains]

• [wiki|Helicase ATP-binding domain] ([protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-like) (aa 40-250) [pubmed|33300032]
• [wiki|Helicase C-terminal domain] ([protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-like) (aa 251-423) [pubmed|33300032]
• Winged helix domain (aa 424-490) [pubmed|33300032]
• Zn²⁺-binding domain (aa 631-749) [pubmed|33300032]

[wiki|Cofactors]

• Zn²⁺ [pubmed|33300032]

Structure

• [PDB|6ZNS] (apo-MrfA) [pubmed|33300032]
• [PDB|6ZNP] (MrfA-DNA) [pubmed|33300032]
• [AF|P50830]

Expression and Regulation

Operons

• genes: [gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A412 (yprA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/412 NBRP B. subtilis, Japan]
• BKE22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT,  downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
• BKK22220 ([gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT,  downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC

References

Reviews

• 35210609

Research papers

• 30379365,33300032