ypdQ

168

similar to RNase HI

locus: BSU_21990

Molecular weight: 14.53 kDa

pI: 5.62

Protein length: 132 aa

Gene length: 399 bp

function: unknown

product: unknown

essential: no

synonyms: ypdQ

List of homologs in different organisms, belongs to COG0328 (Galperin et al., 2021)

Gene

Coordinates: 2,310,419  2,310,817

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The protein

Protein family

• RNase H family (single member, according to UniProt)

[wiki|Domains]

• RNase H domain (aa 1-127) (according to UniProt)

Structure

• [PDB|1GOC] (RNase HI from E. coli, 30% identity) [pubmed|8393706]
• [AF|P54162]

Expression and Regulation

Operons

• genes: [gene|7DD4BB8222B2EFE81C32C5521A29256095B9D6C1|ypdP]-[gene|09343BE8357AB24C79B69816FAE65D161E668F01|ypdQ]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A871 (ypdQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/871 NBRP B. subtilis, Japan]
• BKE21990 ([gene|09343BE8357AB24C79B69816FAE65D161E668F01|ypdQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCTACATATATTTCTGTAG,  downstream forward: _UP4_TAAATCTTGATCATTGGTTC
• BKK21990 ([gene|09343BE8357AB24C79B69816FAE65D161E668F01|ypdQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCTACATATATTTCTGTAG,  downstream forward: _UP4_TAAATCTTGATCATTGGTTC

References

• 22383849,29084857,8393706