yojK

168

similar to macrolide glycosyltransferase

locus: BSU_19420

Molecular weight: 45.44 kDa

pI: 4.92

Protein length: 405 aa

Gene length: 1218 bp

function: unknown

product: unknown

essential: no

synonyms: yojK

List of homologs in different organisms, belongs to COG1819 (Galperin et al., 2021)

Gene

Coordinates: 2,117,051  2,118,268

The protein

Protein family

• UDP-glycosyltransferase family (with [protein|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC] and [protein|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE], according to UniProt)

Structure

• [PDB|7VM0] [pubmed|36091437]
• [PDB|4M83] (from Streptomyces antibioticus, 28% identity)
• [AF|O31853]

Expression and Regulation

Operons

• genes: [gene|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|yojK]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B425 (yojK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1424 NBRP B. subtilis, Japan]
• BKE19420 ([gene|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|yojK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCGGCAGCACCCTTCCT,  downstream forward: _UP4_TAGTGTAAAAGCCTGTTCTT
• BKK19420 ([gene|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|yojK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCGGCAGCACCCTTCCT,  downstream forward: _UP4_TAGTGTAAAAGCCTGTTCTT

References

• 27130432,22383849,31786106,36091437