yodU

yodU
168

5' part of the split gene [gene|240CD6EA3793821F5109252BDEF69C0120E454EF|ypqP] (together with [gene|240CD6EA3793821F5109252BDEF69C0120E454EF|ypqP]), legionaminic acid synthesis, in B. subtilis 168 the gene is disrupted by the [category|SW.5.1.2|SP-beta prophage]

locus
BSU_19810
Molecular weight
15.00 kDa
pI
9.819
Protein length
Gene length
function
legionaminic acid synthesis
product
UDP-NAcGlcA inverting 4,6-dehydratase (N-terminal part)
essential
no
synonyms
yodU, ypqP, spsM

Genomic Context

Categories containing this gene/protein

This gene is a member of the following regulons

Gene
Coordinates
2,151,626 → 2,152,045
Phenotypes of a mutant
the [gene|240CD6EA3793821F5109252BDEF69C0120E454EF|ypqP] mutant of PY79 has a less hydrophobic spore surface [pubmed|32817102]
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The protein
Catalyzed reaction/ biological activity
SpsM catalyzes the C-4/C-6 dehydration of the UDP-GlcNAc to produce UDP-4-keto-6-deoxy-GlcNAc, this is the first reaction of the legionaminic acid biosynthesis pathway [pubmed|32817102]
required for spore crust assembly, legionaminic acid synthesis [pubmed|32817102]
[wiki|Domains]
SpsM: Polysacc_synt_2 domain (Pfam accession number, PF02719) in the 18–296-aa region [Pubmed|25299644]
Structure
[PDB|2GN4] (from Helicobacter pylori, 46% identity) [pubmed|16651261]
Paralogous protein(s)
[protein|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC], (for SpsM)
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU]
description
[Pubmed|22383849]
regulation
expressed late during [wiki|sporulation] in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|25299644,15383836]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|25299644,15383836], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|25299644,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
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[gene|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU]

2024-01-24 03:08:08

ghost

51

1ed0bf5532dffb153093cd9a54f3321649436835

B0C105F0EAA092E93F8DF73CD7A1190BB7F1512A

Biological materials
Mutant
MGNA-A060 (yodU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/60 NBRP B. subtilis, Japan]
BKE19810 (Δ[gene|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC,  downstream forward: _UP4_GTATATTAAGATACTTACTA
BKK19810 (Δ[gene|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCTTATCCTCCAACTC,  downstream forward: _UP4_GTATATTAAGATACTTACTA
References
25299644,15383836,25326298,28535266,16651261,32817102,33803642

F42B27D5D7F3F39828940E377ABCC0730B25EA88

Page visits: 4393

Time of last update: 2024-03-18 07:53:38

Author of last update: Christoph.elfmann