pgpB

168

phosphatidylglycerol phosphate (PGP) phosphatase , has minor undecaprenyl pyrophosphate phosphatase activity

locus: BSU_19650

Molecular weight: 22.64 kDa

pI: 10.14

Protein length: 203 aa

Gene length: 612 bp

function: phospholipid biosynthesis

product: phosphatidylglycerol phosphate (PGP) phosphatase

essential: no

synonyms: yodM

List of homologs in different organisms, belongs to COG0671 (Galperin et al., 2021)

Gene

Coordinates: 2,136,913  2,137,524

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The protein

Catalyzed reaction/ biological activity

• 1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + H₂O --> 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate (according to UniProt)

Protein family

• PA-phosphatase related phosphoesterase family (single member, according to UniProt)

Structure

• [PDB|5JKI] [Pubmed|28168443]
• [AF|O34349]

[wiki|Localization]

• cell membrane (integral) [Pubmed|28168443]

Expression and Regulation

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• GP3652 Δ[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::cat, available in [wiki|Jörg Stülke]'s lab
• GP3680 Δ[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::aphA3, available in [wiki|Jörg Stülke]'s lab
• MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
• BKE19650 ([gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT,  downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
• BKK19650 ([gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT,  downstream forward: _UP4_TAGAGAGAGGAAAACACAGC

FLAG-tag construct

• GP3675 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

References

• 27528508,28168443,26883633