recQ

168

ATP-dependent DNA helicase, acts together with RecJ in replication fork maintenance

Locus: BSU_19220

Molecular weight: 67.18 kDa

Isoelectric point: 5.92

Protein length: 591 aa

Gene length: 1776 bp

Function: replication fork maintenance

Product: ATP-dependent DNA helicase

Essential: no

Synonyms: yocI

Genomic Context

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List of homologs in different organisms, belongs to COG0514 (Galperin et al., 2021)

Gene

Coordinates: 2,094,010 → 2,095,785

The protein

Catalyzed reaction/ biological activity

• unwinds covalently closed ds DNA (in complex with SSB) PubMed
• RecQ controls Topo III (de)catenation activity PubMed
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• helicase family (according to UniProt)

Domains

• Helicase ATP-binding domain (aa 27-196) (according to UniProt)
• Helicase C-terminal domain (aa 216-367) (according to UniProt)
• HRDC domain (aa 513-591) (according to UniProt)

Structure

• 4Q47 (PDB) (RecQ from Deinococcus radiodurans, 40% identity) PubMed
• O34748 (AlphaFold)

Paralogous protein(s)

• RecS

Localization

• associated with the replisome PubMed

Expression and Regulation

Operons

• Genes: bsrB-recQ-yocH

  Description: PubMed

Biological materials

Mutant

• GP3543 (ΔrecQ::kan), available in Jörg Stülke's lab
• MGNA-B414 (yocI::erm), available at the NBRP B. subtilis, Japan
• BKE19220 (ΔrecQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACGTGTAAACCTCGCT,  downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
• BKK19220 (ΔrecQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACGTGTAAACCTCGCT,  downstream forward: _UP4_TAAAAAAACAGAAAAACAAT

References

Reviews

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Original Publications

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