yobL

168

LXG toxin, non-specific metal-dependent RNase

Locus: BSU_19000

Molecular weight: 67.59 kDa

Isoelectric point: 6.5

Protein length: 600 aa

Gene length: 1803 bp

Function: competetion with other bacteria in biofilms

Product: LXG toxin, RNase

Essential: no

E.C.: null

Synonyms: yobL

Genomic Context

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List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

Gene

Coordinates: 2,071,754 → 2,073,556

Phenotypes of a mutant

• the mutant is outcompeted by wild type cells in biofilms PubMed

The protein

Domains

• LXG domain (aa 1-235) (according to UniProt)

Structure

• O34330 (AlphaFold)

Expression and Regulation

Operons

• Genes: yobL-yobK

  Description

  • PubMed

Biological materials

Mutant

• MGNA-A309 (yobL::erm), available at the NBRP B. subtilis, Japan
• BKE19000 (ΔyobL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATTCTCCTTCCTAG,  downstream forward: _UP4_TGAGGTGTTTATATGATTTA
• BKK19000 (ΔyobL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATTCTCCTTCCTAG,  downstream forward: _UP4_TGAGGTGTTTATATGATTTA

References

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