yoaE

168

formate dehydrogenase

locus: BSU_18570

Molecular weight: 76.06 kDa

pI: 7.33

Protein length: 680 aa

Gene length: 2043 bp

function: unknown

product: formate dehydrogenase

essential: no

synonyms: yoaE

List of homologs in different organisms, belongs to COG0243 (Galperin et al., 2021)

Gene

Coordinates: 2,025,400  2,027,442

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The protein

Protein family

• [wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

[wiki|Domains]

• [wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 9-66) (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|2VPW] (from Thermus thermophilus, 25% identity) [pubmed|18536726]
• [AF|C0SP82]

Paralogous protein(s)

• [protein|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]

Expression and Regulation

Operons

• genes: [gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]-[gene|78B66D5B9848D8247D8B9A0DE8923F50F8FCDB46|yoaF]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A836 (yoaE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/836 NBRP B. subtilis, Japan]
• BKE18570 ([gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC,  downstream forward: _UP4_TGAAAAATAATGAAACCCGG
• BKK18570 ([gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC,  downstream forward: _UP4_TGAAAAATAATGAAACCCGG

References

Research papers

• 18536726