yngG

168

3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase

Locus: BSU_18230

Molecular weight: 32.49 kDa

Isoelectric point: 5.75

Protein length: 299 aa

Gene length: 900 bp

Function: mother cell metabolism, leucine utilization

Product: 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) lyase

Essential: no

E.C.: 4.1.3.4

Synonyms: yngG

Genomic Context

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List of homologs in different organisms, belongs to COG0119 (Galperin et al., 2021)

Gene

Coordinates: 1,952,031 → 1,952,930

The protein

Catalyzed reaction/ biological activity

• (S)-3-hydroxy-3-methylglutaryl-CoA --> acetyl-CoA + acetoacetate PubMed

Protein family

• HMG-CoA lyase family (single member, according to UniProt)

Domains

• Pyruvate carboxyltransferase domain (aa 7-274)

Structure

• 1YDO (PDB)
• O34873 (AlphaFold)

Expression and Regulation

Operons

• Genes: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE18230 (ΔyngG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATATGGCATGGTCTTCCTCC,  downstream forward: _UP4_TGATAAAAGGGAGGACGCAT
• BKK18230 (ΔyngG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATATGGCATGGTCTTCCTCC,  downstream forward: _UP4_TGATAAAAGGGAGGACGCAT

References

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