pdhI

168

inhibitor of pyruvate dehydrogenase

locus: BSU_18060

Molecular weight: 10.69 kDa

pI: 3.67

Protein length: 95 aa

Gene length: 288 bp

function: control of pyruvate dehydrogenase activity

product: inhibitor of pyruvate dehydrogenase

essential: no

ec: null

synonyms: yneR

List of homologs in different organisms, belongs to COG4841 (Galperin et al., 2021)

Gene

Coordinates: 1,931,545  1,931,832

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The protein

Catalyzed reaction/ biological activity

• binds to [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA] and [protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB] to inhibit pyruvate dehydrogenase activity [pubmed|36815589]

Protein family

• HesB/IscA family (with [protein|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA], according to UniProt)

Structure

• [AF|Q45063]

Expression and Regulation

Operons

• genes: [gene|ADDEE0B251CBE61892482F15F91543242EDBF2A0|plsY]-[gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]

  description: [Pubmed|22383849]

Biological materials

Mutant

• GP3317 (Δ[gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::''cat''), available in [wiki|Jörg Stülke]'s lab
• BKE18060 ([gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACAGAACACCCCTTTG,  downstream forward: _UP4_TAAAAAAGAACGCACTTCAT
• BKK18060 ([gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACAGAACACCCCTTTG,  downstream forward: _UP4_TAAAAAAGAACGCACTTCAT

Expression vectors

• pGP3580 (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP3588: expression of [gene|5959516D55C9EDBFC46F28C8248D374832895740|pdhI] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab

References

• 36815589