yndE

168

part of the YndD-YndE-YndF germinant receptor of unknown specificity

Locus: BSU_17760

Molecular weight: 40.31 kDa

Isoelectric point: 9.09

Protein length: 363 aa

Gene length: 1092 bp

Function: germination

Product: part of the YndD-YndE-YndF germinant receptor

Essential: no

Synonyms: yndE

Genomic Context

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List of homologs in different organisms, belongs to COG5902 (Galperin et al., 2021)

Gene

Coordinates: 1,909,086 → 1,910,177

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)
• Spore germination protein (SGP) (TC 2.A.3.9) family (according to UniProt)

Structure

• O31809 (AlphaFold)

Paralogous protein(s)

• GerKB, GerBB, GerAB

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yndD-yndE-yndF

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-A023 (yndE::erm), available at the NBRP B. subtilis, Japan
• BKE17760 (ΔyndE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGACTTTATCACCATC,  downstream forward: _UP4_GTCATTTCGAAATGGAGGAA
• BKK17760 (ΔyndE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGACTTTATCACCATC,  downstream forward: _UP4_GTCATTTCGAAATGGAGGAA

References

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