ymfC

168

similar to transcriptional regulator ([wiki|GntR family])

locus: BSU_16810

Molecular weight: 27.75 kDa

pI: 6.4

Protein length: 241 aa

Gene length: 726 bp

function: unknown

product: transcriptional regulator ([wiki|GntR family])

essential: no

synonyms: ymfC

List of homologs in different organisms, belongs to COG2188 (Galperin et al., 2021)

Gene

Coordinates: 1,754,785 → 1,755,510

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The protein

Protein family

• [wiki|GntR family] of transcription factors

[wiki|Domains]

• [wiki|HTH gntR-type domain] (aa 8-76) (according to UniProt)

Structure

• [PDB|2WV0] ([protein|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|nagR], corresponds to aa 83 ... 307 of YmfC, 30% identity)
• [AF|O31761]

Expression and Regulation

Operons

• genes: [gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]

  description: [pubmed|22383849]

• genes: [gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]-[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]

  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B115 (ymfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1114 NBRP B. subtilis, Japan]
• BKE16810 (Δ[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCTCCGTAAAC,  downstream forward: _UP4_TAAAAACGGACGCCTGTATG
• BKK16810 (Δ[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16810 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCTCCGTAAAC,  downstream forward: _UP4_TAAAAACGGACGCCTGTATG

References

• 23504016,20047956