ymcB

168

tRNA methylthiotransferase

locus: BSU_17010

Molecular weight: 58.00 kDa

pI: 6.87

Protein length: 509 aa

Gene length: 1530 bp

function: tRNA maturation

product: tRNA methylthiotransferase

essential: no

synonyms: miaB, ymcB

List of homologs in different organisms, belongs to COG0621 (Galperin et al., 2021)

Gene

Coordinates: 1,772,843  1,774,372

Advertisement

The protein

Catalyzed reaction/ biological activity

• modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA [Pubmed|20472640]
• [sulfur carrier]-SH + AH₂ + N⁶-dimethylallyladenosine³⁷ in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N⁶-dimethylallyladenosine³⁷ in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H⁺ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• methylthiotransferase family (with [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV], according to UniProt)

[wiki|Domains]

• MTTase N-terminal domain (aa 66-184) (according to UniProt)
• [wiki|TRAM domain] (aa 440-503) (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|4JC0] (from Thermotoga maritima, 33% for aa 70 ... 504) [pubmed|23542644]
• [AF|O31778]

Paralogous protein(s)

• [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]-[gene|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• GP1109 (spc), available in [wiki|Jörg Stülke]'s lab
• BKE17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT,  downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
• BKK17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT,  downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA

References

• 20472640,23542644