rnpM

rnpM
168

modulator of RNase P

locus
BSU_16610
Molecular weight
10.27 kDa
pI
10.09
Protein length
Gene length
function
control of tRNA maturation
product
modulator of RNase P
essential
no
synonyms
ylxR, ymxB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2740 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,733,410 → 1,733,685
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The protein
Catalyzed reaction/ biological activity
binds to the RNA component ([protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]) of RNase P to limit its activity [pubmed|38050972]
Structure
[PDB|1G2R] (from ''Streptococcus pneumoniae'', 47% identity) [Pubmed|11679764]
[AF|P32728]
Kinetic information
KD ([protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]-[protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] interaction): 460 pM [pubmed|38050972]
Expression and Regulation
Operons
genes
[gene|9F6C447900410BF64409E5C41A11C76968DADAFA|ylxS]-[gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]-[gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]-[gene|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA]-[gene|378B77412D0DEA220A97DEC246410509D9B74D46|infB]-[gene|24835CE6CD2E1F183DB9121F29A63101718B5DF9|ylxP]-[gene|B3119A40393A288EF34FC3E9839B6E40AC6A6EA0|rbfA]
description
[Pubmed|8491709]
regulation
autoregulation [wiki|NusA] [pubmed|27571753]
induced by glucose [pubmed|30355672]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
[protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]: attenuation, [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] stimulates termination [Pubmed|27571753], in [regulon|protein:8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8491709], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528,26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
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[gene|9F6C447900410BF64409E5C41A11C76968DADAFA|ylxS]→[gene|B3119A40393A288EF34FC3E9839B6E40AC6A6EA0|rbfA]

2024-03-10 09:13:33

Jstuelk

90

019B939D583142A5395482C3EECB5B469F354080

A30223501809D45C115085980399F87C5C5A0442

Biological materials
Mutant
GP3753 ([gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]::''kan''), available in [wiki|Jörg Stülke]'s lab
MGNA-B078 (ΔylxR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1077 NBRP B. subtilis, Japan]
BKE16610 (Δ[gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTAAAGTCACCTCTTAT,  downstream forward: _UP4_CTGGCGGAAAAGGTGAAAAA
BKK16610 (Δ[gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16610 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACCTAAAGTCACCTCTTAT,  downstream forward: _UP4_CTGGCGGAAAAGGTGAAAAA
Expression vectors
pGP3617 expression of [gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3618 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in [wiki|Jörg Stülke]'s lab
pGP3647: expression of [gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
pGP3650: (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP3651: (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
36882621
Original Publications
11948165,8491709,11679764,30355672,31118925,33101263,34906218,27571753,32983026,38050972

F4097349A563503468A2A14F062AEAC532C7917A

Page visits: 4796

Time of last update: 2024-03-29 04:46:40

Author of last update: Jstuelk