rsmH

rsmH
168

SAM-dependent 16S rRNA m4C1402 methyltransferase

locus
BSU_15140
Molecular weight
35.15 kDa
pI
6.03
Protein length
Gene length
function
fine-tuning of the ribosomal decoding center
product
Ribosomal RNA small subunit methyltransferase H
essential
no
ec
2.1.1.199
synonyms
mraW, yllC, ylxA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,580,622  1,581,557
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The protein
Catalyzed reaction/ biological activity
cytidine1402 in 16S rRNA + S-adenosyl-L-methionine --> H+ + N4-methylcytidine1402 in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
Structure
[PDB|3TKA] (from ''Escherichia coli'', 46% identity) [pubmed|22561317]
[PDB|1N2X] (from ''Thermotoga maritima'', 41% identity, 70% similarity) [Pubmed|12824489]
[AF|Q07876]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
description
[Pubmed|8636036,8244929]
regulation
constitutively expressed [Pubmed|15758244]
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[gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]→[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]

2024-02-07 05:18:26

Jstuelk

84

d5321fe6b1e9387ca670f35171261c39867c1f3d

9D42077BFFD1B3A740698D26FB54D7B31E3FD0C7

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-B130 (ylxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1129 NBRP B. subtilis, Japan]
1A818 ( ''mraW''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A818&Search=1A818 BGSC]
BKE15140 ([gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTGGTCCCACC,  downstream forward: _UP4_TAACGAAACGCGAACGCAAA
BKK15140 ([gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTGGTCCCACC,  downstream forward: _UP4_TAACGAAACGCGAACGCAAA
References
Reviews
36411352
Original Publications
8636036,12824489,26883633,19965768,22561317,10572301

1F894D6895CE13AA006CFE13A702E46C568F6E79

Page visits: 3897

Time of last update: 2024-03-29 04:46:35

Author of last update: Jstuelk