yloM

168

similar to RNA-binding Sun protein, similar to S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

locus: BSU_15740

Molecular weight: 50.11 kDa

pI: 6.95

Protein length: 447 aa

Gene length: 1344 bp

function: rRNA modification

product: S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

essential: no

synonyms: yloM, sun

List of homologs in different organisms, belongs to COG0781 (Galperin et al., 2021)

Gene

Coordinates: 1,647,939  1,649,282

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The protein

Catalyzed reaction/ biological activity

• cytidine⁹⁶⁷ in 16S rRNA + S-adenosyl-L-methionine --> 5-methylcytidine⁹⁶⁷ in 16S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• [wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

Structure

• [PDB|1SQF] (''E. coli'' Fmu protein, complex with SAM) [Pubmed|14656444]
• [AF|P94464]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description: [Pubmed|16964327]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

  description

  • [pubmed|22383849]

Biological materials

Mutant

• MGNA-B373 (yloM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1372 NBRP B. subtilis, Japan]
• BKE15740 ([gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC,  downstream forward: _UP4_AGCATGAGAAAGAAGGGATA
• BKK15740 ([gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC,  downstream forward: _UP4_AGCATGAGAAAGAAGGGATA

References

• 36815589 16964327,14656444