ylaG

168

similar to GTP-binding elongation factor

locus: BSU_14770

Molecular weight: 68.21 kDa

pI: 4.97

Protein length: 612 aa

Gene length: 1839 bp

function: unknown

product: unknown

essential: no

synonyms: ylaG, bipA, typA

List of homologs in different organisms, belongs to COG1217 (Galperin et al., 2021)

Gene

Coordinates: 1,546,121  1,547,959

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The protein

Catalyzed reaction/ biological activity

• in E. coli, the corresponding protein (BipA) incorporates ribosomal protein [protein|20485A785D3AF82889F2018D3EA5B5FA33E64E95|L6] into the nascent large ribosomal subunit [pubmed|30305394]

Protein family

• [wiki|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
• [wiki|Classic translation factor GTPase family] (according to UniProt)

[wiki|Domains]

• [wiki|tr-type G domain] (aa 5-200) (according to UniProt)

Structure

• [PDB|4ZCM] (from E. coli, 55% identity) [pubmed|26163516]
• [AF|O07631]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]-[gene|8DE3D48909BDBD2091432047E79063E9EEE63115|ylaH]

  description: [pubmed|22383849]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• MGNA-A536 (ylaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/536 NBRP B. subtilis, Japan]
• BKE14770 ([gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATCTCCTAAAA,  downstream forward: _UP4_TAATCTCGTGCAGAATTTGA
• BKK14770 ([gene|2D0A95A37214E9BF58E2DE732DE9F32AAF9C1CD3|ylaG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATCTCCTAAAA,  downstream forward: _UP4_TAATCTCGTGCAGAATTTGA

References

• 36815589,36815589,11948165,12399512,30305394,26163516