ykoV

168

DNA-end-binding protein Ku, non-homologous end joining DNA repair

locus: BSU_13410

Molecular weight: 34.91 kDa

pI: 7.21

Protein length: 311 aa

Gene length: 936 bp

function: non-homologous end joining DNA repair, repair of gapped DNA substrates

product: DNA-end-binding protein Ku

essential: no

synonyms: ykoV

List of homologs in different organisms, belongs to COG1273 (Galperin et al., 2021)

Gene

Coordinates: 1,406,357 → 1,407,292

Phenotypes of a mutant

• sensitivity to ionizing radiation in the stationary phase  [Pubmed|12215643]
• sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]
• a ''[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]-[gene|127CB0930A2D96B18D9261180028818F158C68CB|ligD]'' double mutant is sensitive to radiation [Pubmed|24123749]
• reduced resistance towards electron beams [pubmed|31948638]

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The protein

Catalyzed reaction/ biological activity

• stimulation of [protein|127CB0930A2D96B18D9261180028818F158C68CB|ligD] non-homologous end-joining activity [Pubmed|23691176,26961308]
• binds at the DNA double-strand breaks and recruits the ligase [protein|search|LigD ]to seal the break [pubmed|15778718,16518468]

Protein family

• prokaryotic Ku family (single member, according to UniProt)

[wiki|Domains]

• KU domain (aa 26-210) (according to UniProt)

Structure

• [AF|O34859]

[wiki|Localization]

• associates with the nucleoid during germination [Pubmed|16497325]

Expression and Regulation

Operons

• genes: [gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]-[gene|127CB0930A2D96B18D9261180028818F158C68CB|ligD]

  description: [Pubmed|16497325]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG], [wiki|SpoVT]) [Pubmed|16497325]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: activation, [Pubmed|16497325], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-A779 (ykoV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/779 NBRP B. subtilis, Japan]
• BP809 (Δ[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]::''cat'') available in [wiki|Juan Alonso]'s and [wiki|Jörg Stülke]'s labs [pubmed|16780573]
• BP141 (Δ''[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]-[gene|127CB0930A2D96B18D9261180028818F158C68CB|ligD]''::''kan'') available in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s labs [pubmed|30863384]
• BP142 (Δ''[gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]-[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]-[gene|127CB0930A2D96B18D9261180028818F158C68CB|ligD]''::''kan'') available in [wiki|Fabian Commichau]'s lab
• BKE13410 (Δ[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTAGTAACTTATGGG,  downstream forward: _UP4_AAAGCCTCCGGCACATCATA
• BKK13410 (Δ[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTAGTAACTTATGGG,  downstream forward: _UP4_AAAGCCTCCGGCACATCATA

References

Reviews

• 11591342,11719239,17938628,22933559,30746801

Original Publications

• 16497325,11483577,23691176,12215643,17293412,24123749,25355514,26961308,15778718,16518468,29234047,30976810,31948638