ykoT

168

similar to lipoteichoic acid glycosyltransferase

locus: BSU_13390

Molecular weight: 38.36 kDa

pI: 9.34

Protein length: 337 aa

Gene length: 1014 bp

function: unknown

product: unknown

essential: no

synonyms: ykoT

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 1,403,479 → 1,404,492

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The protein

Protein family

• [wiki|glycosyltransferase 2 family] (according to UniProt)

Structure

• [PDB|5EKP] (from Synechocystis sp., 38% identity) [pubmed|26729507]
• [AF|O34755]

Paralogous protein(s)

• [protein|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]
• [protein|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|ykcC], (39%)

[wiki|Localization]

• cell membrane (heterogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|6B66E6E138ED70318E5911F310953D8F09FA8719|ykoS]-[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]

  description: [Pubmed|22383849]

  regulation

  • expressed late during [wiki|sporulation] in the forespore ([wiki|SigG], [wiki|SpoVT]) [Pubmed|26577401]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|26577401], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|26577401], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-A777 (ykoT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/777 NBRP B. subtilis, Japan]
• BKE13390 (Δ[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGACTGTTTCAATCGATATC,  downstream forward: _UP4_TAAACACGGAAAGAGCTGAC
• BKK13390 (Δ[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGACTGTTTCAATCGATATC,  downstream forward: _UP4_TAAACACGGAAAGAGCTGAC

References

• 21630458,16479537,22383849,26577401,27185829,26729507