yknT

168

spore coat protein

locus: BSU_14250

Molecular weight: 37.51 kDa

pI: 7.16

Protein length: 321 aa

Gene length: 966 bp

function: protection of the spore

product: spore coat protein

essential: no

synonyms: yknT, cse15

List of homologs in different organisms

Gene

Coordinates: 1,494,403  1,495,368

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The protein

Structure

• [PDB|1KNV] (Geobacillus stearothermophilus)
• [AF|O31700]

[wiki|Localization]

• outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|A922E599E67C4B93046E7F05F25CB32F9B14FAF0|yknT]

  description: [Pubmed|8990290]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|15699190,15383836,8990290]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: activation, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,8990290], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• MGNA-A056 (yknT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/56 NBRP B. subtilis, Japan]
• BKE14250 ([gene|A922E599E67C4B93046E7F05F25CB32F9B14FAF0|yknT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGAACCATCTCCCTCA,  downstream forward: _UP4_TAAAAAAACTGCTTGAGCGA
• BKK14250 ([gene|A922E599E67C4B93046E7F05F25CB32F9B14FAF0|yknT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCGAACCATCTCCCTCA,  downstream forward: _UP4_TAAAAAAACTGCTTGAGCGA

References

• 15699190,8990290,15383836,22171814,28870294