ykhA

168

similar to acyl-CoA thioesterase

locus: BSU_13030

Molecular weight: 19.15 kDa

pI: 7.79

Protein length: 172 aa

Gene length: 519 bp

function: unknown

product: unknown

essential: no

synonyms: ykhA

List of homologs in different organisms, belongs to COG1607 (Galperin et al., 2021)

Gene

Coordinates: 1,372,035 → 1,372,553

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The protein

Protein family

• acyl coenzyme A hydrolase family (single member, according to UniProt)

[wiki|Domains]

• HotDog ACOT-type domain (aa 10-122) (according to UniProt)

Structure

• [PDB|1Y7U] (from ''Bacillus Cereus'', 59% identity)
• [PDB|3SPS] (from ''Bacillus halodurans'', 49% identity, 80% similarity)
• [AF|P49851]

Expression and Regulation

Operons

• genes: [gene|B6EAAEFF8BBE8F8381F3999C6D1E5EFEBC90B0BA|ykhA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A744 (ykhA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/744 NBRP B. subtilis, Japan]
• BKE13030 (Δ[gene|B6EAAEFF8BBE8F8381F3999C6D1E5EFEBC90B0BA|ykhA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTCTCCCCTTTGA,  downstream forward: _UP4_TGAACGTTAAACCCAAAACA
• BKK13030 (Δ[gene|B6EAAEFF8BBE8F8381F3999C6D1E5EFEBC90B0BA|ykhA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTCTCCCCTTTGA,  downstream forward: _UP4_TGAACGTTAAACCCAAAACA

References

Research papers

• 28516784