bstD

168

bacillithiol S-transferase

locus: BSU_12410

Molecular weight: 17.65 kDa

pI: 5.81

Protein length: 154 aa

Gene length: 465 bp

function: unknown

product: bacillithiol S-transferase

essential: no

synonyms: bstD, yjoA

List of homologs in different organisms, belongs to COG2318 (Galperin et al., 2021)

Gene

Coordinates: 1,313,840 → 1,314,304

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The protein

Protein family

• [wiki|S-transferase-like (STL) superfamily] [pubmed|29451913]
• [wiki|DinB family] (according to UniProt)

Structure

• [PDB|3DKA]
• [AF|O34334]

Modification

• phosphorylation on Tyr-150 [Pubmed|17218307]

Expression and Regulation

Operons

• genes: [gene|B22075CDA06A94557DF83B3D29B7BF83E2A51EA5|bstD]

  description: [Pubmed|22383849]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-A356 (yjoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/356 NBRP B. subtilis, Japan]
• BKE12410 (Δ[gene|B22075CDA06A94557DF83B3D29B7BF83E2A51EA5|bstD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGATCTCCTCCATAT,  downstream forward: _UP4_TAGCGTAAACAAGAAAAAAG
• BKK12410 (Δ[gene|B22075CDA06A94557DF83B3D29B7BF83E2A51EA5|bstD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGATCTCCTCCATAT,  downstream forward: _UP4_TAGCGTAAACAAGAAAAAAG

References

• 12884008,24728941,17218307,22383849,29451913,36191235