yitJ

168

bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase

Locus: BSU_11010

Molecular weight: 67.75 kDa

Isoelectric point: 5.34

Protein length: 612 aa

Gene length: 1839 bp

Function: methionine biosynthesis, tetrahydrofolate interconversion

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase

Essential: no

E.C.: 2.1.1.13

Synonyms: yitJ

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Biosynthesis/ acquisition of amino acids, Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0685 (Galperin et al., 2021)

Gene

Coordinates: 1,178,757  1,180,595

The protein

Catalyzed reaction/ biological activity

S-methyl-L-methionine + L-homocysteine --> 2 L-methionine (according to UniProt)
5-methyltetrahydrofolate + NAD(P)⁺ --> 5,10-methylenetetrahydrofolate + NAD(P)H (according to UniProt)

Protein family

C-terminal part: methylenetetrahydrofolate reductase family (single member, according to UniProt)

Domains

Hcy-binding domain (aa 1-280) (according to UniProt)

Cofactors

FAD (according to UniProt)
Zn²⁺ (according to UniProt)

Structure

1Q7M (PDB) (the methionine synthase domain, from Thermotoga maritima, 31% identity) PubMed
6FNU (PDB) (the methylene THF reductase domain, from Saccharomyces cerevisiae, 25% identity)
O06745 (AlphaFold)

Localization

membrane associated PubMed

Expression and Regulation

Operons

Genes: yitJ

Description

PubMed

Regulation

induced by methionine starvation (S-box) PubMed
the S-box RNA is degraded by RNase Y PubMed

Regulatory mechanism

S-box: RNA switch, PubMed, in S-box

Biological materials

Mutant

MGNA-B195 (yitJ::erm), available at the NBRP B. subtilis, Japan
BKE11010 (yitJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT,  downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
BKK11010 (yitJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT,  downstream forward: _UP4_TAATTTCCAAAAGACTGCCT

References

Other publications

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The [[protein|S-box]]

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